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目的观察芍药苷给药后对小鼠哮喘模型气道炎症趋化因子及受体的影响。方法用卵蛋白(OVA)致敏和激发建立小鼠哮喘模型;ELISA法检测血清IL-6、TNF-α水平及支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中卵蛋白特异性Ig E(OVA-Ig E)和趋化因子CCL19、CCL21水平;RT-PCR法检测肺组织中趋化因子受体CCR7mRNA表达;Western blot检测肺组织CCR7及核转录因子-κB(nuclear factor-κB,NF-κB)蛋白表达。结果芍药苷干预组小鼠血清IL-6、TNF-α水平显著下降;BALF中OVA-Ig E和CCL19、CCL21水平显著降低;肺组织CCR7mRNA、CCR7及NF-κB蛋白表达明显减少。结论芍药苷对哮喘模型小鼠气道炎症趋化因子CCL19/CCL21及其受体CCR7具有显著的抑制作用。
Objective To observe the effect of paeoniflorin on chemokines and receptors of airway inflammation in mouse asthma model. Methods The mouse asthma model was established by sensitization and challenge with ovalbumin (OVA). Serum levels of IL-6 and TNF-α and the level of ovalbumin-specific IgE (OVA) in bronchoalveolar lavage fluid (BALF) -Ig E) and chemokines CCL19 and CCL21. The expression of chemokine receptor CCR7 mRNA in lung tissue was detected by RT-PCR. The expression of CCR7 and NF-κB )Protein. Results Serum levels of IL-6 and TNF-α in paeoniflorin-treated mice decreased significantly. The levels of OVA-Ig E, CCL19 and CCL21 in BALF were significantly decreased. The expressions of CCR7 mRNA, CCR7 and NF-κB in lung tissue were significantly decreased. Conclusion Paeoniflorin has a significant inhibitory effect on airway inflammation chemokine CCL19 / CCL21 and its receptor CCR7 in asthmatic mice.