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目的观察前列腺癌和前列腺增生中CD4+CD25+调节性T细胞在前列腺癌和前列腺增生中的频率分布,并探讨其与组织分化的关系,为临床诊断、治疗前列腺增生和前列腺癌提供理论依据。方法选择青岛市第八人民医院泌尿外科就诊并手术的前列腺患者90例,采用放射免疫法测定所有患者术前血清PSA水平,应用伊红-苏木素(HE)染色观察前列腺组织形态学的改变,利用多色流式分析术检测患者外周血CD4+CD25+调节性T细胞的频率。结果高分化组可见腺体大小和形态较不规则,可见核分裂相和深染核。中分化组可见腺体结构不完整,有公壁现象或融合成筛状。低分化组可见大量浸润生长的低分化细胞,腺体结构消失。各组之间血清PSA水平无显著性差别(P>0.05)。与前列腺增生组比较,前列腺癌组的血清PSA水平明显增高,差别有极显著意义(P<0.01)。前列腺癌患者外周血CD4+CD25+T细胞所占比例较前列腺增生患者明显升高,并且与组织分化程度密切相关。结论 PSA值与前列腺癌临床分期之间无相关性。前列腺癌患者外周血CD4+CD25+T细胞所占比例较前列腺增生患者明显升高,且与组织分化程度密切相关。前列腺癌患者体内CD4+CD25+T细胞比例上升可能是导致免疫抑制的原因之一,并可作为评价前列腺癌恶性程度的指标之一。
Objective To observe the frequency distribution of CD4 + CD25 + regulatory T cells in prostatic cancer and benign prostatic hyperplasia in prostate cancer and benign prostatic hyperplasia, and to explore the relationship between CD4 + CD25 + regulatory T cells and histological differentiation in order to provide theoretical basis for clinical diagnosis, treatment of benign prostatic hyperplasia and prostate cancer. Methods Ninety patients with prostate cancer treated and operated in Department of Urology, Eighth People ’s Hospital of Qingdao were enrolled in this study. Preoperative serum PSA levels were measured by radioimmunoassay. Eosin - hematoxylin (HE) staining was used to observe the histological changes of the prostate. Multicolor flow cytometry analysis of peripheral CD4 + CD25 + regulatory T cells in patients with the frequency. Results showed that the size and shape of glands in the well-differentiated group were irregular, showing mitotic and deep-stained nuclei. Differentiation group shows that the structure of the gland is not complete, there are public wall phenomenon or into a mesh-like phenomenon. Poorly differentiated group showed a large number of poorly differentiated cells infiltrating growth, glandular structure disappeared. There was no significant difference in serum PSA between groups (P> 0.05). Compared with benign prostatic hyperplasia group, the serum PSA level of prostate cancer group was significantly higher, the difference was extremely significant (P <0.01). The proportion of CD4 + CD25 + T cells in peripheral blood of patients with prostate cancer was significantly higher than that of benign prostatic hyperplasia patients, and was closely related to the degree of tissue differentiation. Conclusion There is no correlation between PSA and clinical stage of prostate cancer. The proportion of CD4 + CD25 + T cells in peripheral blood of patients with prostate cancer is significantly higher than that of benign prostatic hyperplasia patients, and is closely related to the degree of tissue differentiation. Prostate cancer patients with increased proportion of CD4 + CD25 + T cells may be one of the reasons leading to immune suppression, and can be used as an indicator of the degree of malignancy of prostate cancer.