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目的 检测前列腺癌组织中 p5 3基因突变的发生率 ,探讨其与前列腺癌发生的关系。 方法 应用多聚酶链反应 -限制性片段长度多态性方法 ,检测前列腺癌组织中 p5 3基因第 4号外显子 72位密码子的点突变。 结果 前列腺癌 DNA样本中检出 4号外显子 p5 3基因点突变 44 % ,其中杂合性突变 36 % ,纯合性突变 8%。对照组前列腺良性增生未见 p5 3基因变化。 结论 前列腺癌发生与 p5 3基因突变有一定关系 ,应用 PCR和 Bstu I酶切多态性分析 ,对检测前列腺癌 p5 3基因第 4号外显子 72位编码的点突变是一较特异的方法。
Objective To detect the incidence of p53 gene mutation in prostate cancer tissues and to investigate its relationship with prostate cancer. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the point mutation of codon 72 of exon 4 of p53 gene in prostate cancer tissues. Results The point mutation of p53 in exon 4 was detected in 44% of prostate cancer DNA samples, of which the heterozygous mutation was 36% and the homozygous mutation was 8%. There was no p5 3 gene change in the benign prostatic hyperplasia of the control group. Conclusion There is a certain relationship between the occurrence of prostate cancer and mutation of p53 gene. Using PCR and Bstu I polymorphism analysis, point mutations encoding exon 72 of prostate cancer p53 gene are a more specific method.