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目的:通过动物实验,研究小儿喘咳液合苍耳子散抗哮喘气道重塑作用及机理。方法:将135只大鼠随机分成空白组、模型组、西药组、喘咳液组、大、中、小剂量及合方大、中、小剂量组9个组,每组15只,进行哮喘气道重塑模型制备,分别采用不同的干预方案,观察各组大鼠过敏性鼻炎叠加量化积分、血清TGF-β1表达及对肺支气管形态的影响。结果:喘咳液大剂量组及合方大剂量组与模型组相比,引喘70 d后过敏性鼻炎叠加积分下降,差异有显著性(P<0.05);喘咳液大剂量组及合方大剂量组与空白组相比,TGF-β1水平下降,差异有显著性(P<0.05);西药组、喘咳液各剂量组及合方各剂量组与模型组相比,TGF-β1水平明显下降,差异有非常显著性(P<0.01);病理检查可见小儿喘咳液合苍耳子散能使气道重塑大鼠支气管炎症明显改善,并减轻气道平滑肌增厚及管腔狭窄。结论:小儿咳喘液及合方可改善过敏性鼻炎症状,其治疗哮喘的机制之一可能是通过降低TGF-β1的水平,从而抑制平滑肌增殖,减少胶原合成及沉积、形成纤维化,减轻哮喘气道炎症,预防气道重塑的发生。
OBJECTIVE: To study the anti-asthmatic airway remodeling effect and its mechanism in children with astragalus mongholicus through animal experiments. Methods: 135 rats were randomly divided into blank group, model group, western medicine group, asthma cough liquid group, large, medium and small dose and large, medium and small dose group of 9 groups, 15 in each group, asthma The airway remodeling model was prepared. Different intervention programs were used to observe the quantitative integral of allergic rhinitis, the expression of serum TGF-β1 and the effect on bronchial morphology of the lung in each group. Results: Compared with the model group, the cumulative score of hypersensitive rhinitis in asthmatic group was significantly lower than that of the model group (P <0.05) Compared with the blank group, the TGF-β1 level decreased significantly in the Fangda dose group (P <0.05); Compared with the model group, the TGF-β1 (P <0.01). Pathological examination showed that children’s asthma cough solution combined with xanthium powder could significantly improve the bronchial inflammation in airway remodeling rats and alleviate airway smooth muscle thickening and lumen narrow. Conclusion: Pediatric Kechuan Liquid and its combination can improve the symptoms of allergic rhinitis. One of the mechanisms of its treatment for asthma may be to reduce the level of TGF-β1, thereby inhibiting smooth muscle proliferation, reducing collagen synthesis and deposition, forming fibrosis, reducing asthma Airway inflammation, prevention of airway remodeling.