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用TAIL-PCR的方法获得了A1-412突变体的T-DNA侧翼基因组序列,测序结果表明,外源T-DNA插入到稻瘟病菌G蛋白γ亚基基因MGG1(MagnaporthegriseaGproteinGamma亚基)的启动子区域.MGG1编码93个氨基酸,具有典型的G蛋白γ亚基结构域(GGL)和C末端CAAX框,并与其他丝状真菌G蛋白γ亚基有较高的一致性.外加cAMP能诱导部分A1-412分生孢子形成附着胞,但这些附着胞的形态不正常,不能穿透洋葱内表皮或水稻叶片.另外,A1-412与相对应交配型的菌株杂交时几乎不产生子囊壳.将MGG1基因重新导入A1-412突变体可部分恢复上述表型.这些结果表明,G蛋白γ亚基MGG1可能涉及稻瘟病菌形态分化、有性生殖、致病性等方面的调节作用.
The T-DNA flanking genomic sequence of A1-412 mutant was obtained by TAIL-PCR. The sequencing results showed that the exogenous T-DNA was inserted into the promoter of Magnaporthe grisea G protein Gamma subunit MGG1 Region.MGG1 encodes 93 amino acids with typical G-protein gamma subunit (GGL) domain and C-terminal CAAX box and has high homology with other filamentous fungi G protein gamma subunit.CAMP can induce partial A1-412 conidia formed attachment cells, but these attached cells morphologically abnormal, can not penetrate the onion epidermis or rice leaves.In addition, A1-412 and the corresponding mating type strains when hybridized almost no ascithelium will The re-introduction of MGG1 gene into A1-412 mutant partially restored the above phenotypes.These results indicated that G protein gamma subunit MGG1 may be involved in the regulation of the morphogenic differentiation, sexual reproduction and pathogenicity of Magnaporthe grisea.