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目的观察和探讨COX-2选择性抑制剂NS398对卵巢癌细胞系CAOV3增殖抑制作用及对CTL细胞体外免疫杀伤作用的影响。方法放射免疫法测定CAOV3细胞及经NS398处理的CAOV3细胞培养上清液中的PGE2含量,流式细胞仪分析CTL细胞表型,MTT比色法检测CTL细胞杀伤活性。结果 NS398能抑制CAOV3细胞分泌PGE2;经NS398处理的CAOV3刺激诱导产生CTL细胞对CAOV3细胞的杀伤活性显著增加。结论 NS398可能成为卵巢癌预防与治疗的新靶点。
Objective To observe and explore the effect of COX-2 selective inhibitor NS398 on the proliferation of ovarian cancer cell line CAOV3 and its effect on CTL cells in vitro. Methods Radioimmunoassay was used to determine the content of PGE2 in CAOV3 cells and CAOV3 cell culture supernatants treated with NS398. The CTL cell phenotypes were analyzed by flow cytometry. The cytotoxic activity of CTLs was detected by MTT assay. Results NS398 could inhibit the secretion of PGE2 in CAOV3 cells. The cytotoxic activity of CTL cells induced by NS398-treated CAOV3 cells on CAOV3 cells was significantly increased. Conclusion NS398 may be a new target for the prevention and treatment of ovarian cancer.