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目的：研究选择性COX-2抑制剂塞来昔布对低氧模拟剂氯化钴诱导的胃癌细胞株SGC-7901 OCT-4表达的影响。方法以胎牛血清加RPMI 1640培养基培养细胞作正常对照，分别应用150μmol/L氯化钴联合0μmol/L、25μmol/L、75μmol/L的塞来昔布作用于SGC-7901细胞48h,应用RT-PCR技术检测细胞中OCT-4mRNA表达。结果与正常对照组比较,150μmol/L氯化钴刺激48h,可显著提高细胞OCT-4mRNA 的表达(P<0.05),塞来昔布和氯化钴联合作用48h,细胞OCT-4 mRNA表达降低(P<0.05),并呈剂量依赖性。结论 COX-2抑制剂塞来昔布可抑制氯化钴诱导的OCT-4表达,这可能是其抗肿瘤的机制之一。“,”Ami : To study the effects of COX-2-selected inhibitor Celecoxib on the expression of OCT-4 in human gastric cancer cellline SGC-7901 cells induced by hypox iamimic cobalt chloride. Methods: SGC-7901 cells stimulated by cobalt chloride(150μmol/L) or combined with 0μmol/L,25μmol/L,and75μmol/L Celecoxib for 48h,were subjected to determine the expression of OCT-4 mRNA using RT-PCR. Results:Compared with the SGC-7901 cells without any induction, 150μmol/L cobalt chloride stimulated the expression of OCT-4mRNA and in SGC-7901 cells (P<0.05), and Celecoxib prevented the cobalt chloride-sim ulated up-regulation of OCT-4mRNA in a do se-dependent manner(P<0.05). Conclusion:Celecoxib could inhibit the high expression of OCT-4mRNA in SGC-7901 cells induced by hypoxia, which may be one of its antiangiogenesism echanisms.