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类风湿因子（RF）是一种以变性IgG为靶抗原的自身抗体,主要分为IgM-RF、IgG-RF和IgA-RF等三种类型,多存在于类风湿性关节炎附则患者血清和关节液中。由于RF在其他自身免疫病患者和一些病毒性感染、细菌感染性疾病及一部分正常人体内均可测出低效价的RF,给RA的诊断带来了困难。因此,应用RF滴度对RA诊断将会增强特异性和准确性。本文总结了自 1995年1月至1996年 6月来我院就诊的742例明确诊断为风湿病患者血清RF滴度的测定结果,并比较了RA患者和非RA患者血清中RF滴度,现报告如下。1 材料和方法1.1实验对象本文选择的实验组病例均系本院风湿科按美国抗风湿病学会（ARA,1982）诊断标准确诊病冽,其中类风湿性关节炎（RA）314例,系统性红斑狼疮（SLE）183例,干燥综合征（SS）163例,混合结缔组织病（MCTD）29例,皮肌炎和多肌炎（DM/PM）36例,进行性系统性硬化症（PSS）17例。对照组50例,均为本院体检健康者。 本文统计资料均为各患者一次检测结果。1.2实验方法IgM-RP乳胶凝集试剂由上海荣盛试剂公司提供。按说明书操作。 血标本均为当日新鲜血液,常规法分离血清后按1:20、1:40、1:80……倍比稀释直到RF测定转阴性为止。2结果2.1各类风湿病RF滴度测定结果见表1。2.2 RF滴度 RA组最高,滴度在1:80以上,占
Rheumatoid factor (RF) is a kind of autoantibodies with denatured IgG as the target antigen, which is mainly divided into three types: IgM-RF, IgG-RF and IgA-RF and is mainly present in the serum of patients with rheumatoid arthritis Joint fluid. Because RF can detect low-potency RF in other autoimmune diseases and some viral infections, bacterial infectious diseases and some normal human body, it brings difficulties to the diagnosis of RA. Therefore, using RF titers for RA diagnosis will enhance specificity and accuracy. This article summarizes the results of the determination of the serum RF titer of 742 patients diagnosed as rheumatism from January 1995 to June 1996 and compared the serum RF titer in patients with RA and non-RA The report is as follows. 1 Materials and Methods 1.1 Subjects The experimental group selected in this article are rheumatoid arthritis hospital in our hospital (ARA, 1982) diagnostic criteria for diagnosis of disease, including rheumatoid arthritis (RA) 314 cases of systemic 183 cases of systemic lupus erythematosus (SLE), 163 cases of Sjogren’s syndrome (SS), 29 cases of mixed connective tissue disease (MCTD), 36 cases of dermatomyositis and polymyositis (DM / PM) ) 17 cases. The control group of 50 cases, all of our hospital physical examination. This statistical data are the results of a test for each patient. 1.2 Experimental Methods IgM-RP latex agglutination reagent provided by Shanghai Rongsheng Reagent Company. Follow the instructions. Blood samples were fresh blood the same day, the conventional method of separation of serum 1: 20,1: 40,1: 80 ...... ratio than the dilution until the RF measured negative negative. 2 Results 2.1 various types of rheumatic RF titer determination results in Table 1.2.2 RF titer RA group highest titers in the 1:80 or more, accounting for