【摘 要】
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Objective: The purpose of this study was to investigate the efect of Laggera alata flavonen (LAF) on the inhibit-ing efect of human ovarian cancer HO-8910 cels
【机 构】
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Department of 0bstetrics and Gynecology,Department of Information
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Objective: The purpose of this study was to investigate the efect of Laggera alata flavonen (LAF) on the inhibit-ing efect of human ovarian cancer HO-8910 cels proliferation and its possible mechanism in vitro.Methods: Human ovarian cancer HO-8910 cels were culturedin vitro. Inhibitory efect of LAF on the viability of HO-8910 cels was evaluated by the MTT assay. Apoptotic efect of diferent concentrations of LAF on HO-8910 cels was assessed by AO/EB staining and FCM with propidium iodide (PI) staining. Expression of proteins related to apoptosis was analyzed by Western blot.Results: LAF significantly inhibited the viability of HO-8910 cels proliferation in a dose-dependent and time-dependent manner, there were statistical significance compared with NS group (P < 0.05), and the IC50 was 4.28 μg/mL for 48 h. The cels treated with LAF showed typical morphological change and apoptotic rate increased by FCM in a dose-dependent, and there was notable dif-ference compared with NS group (P < 0.05). Western blot showed that expression of Fas, caspase-8, tBid and Cyto-c proteins were up-regulated after treatment with LAF for 48 h in a concentration dependent.Conclusion: LAF could inhibit HO-8910 cels proliferation and induce apoptosis, which may be through the pathway of death receptorin vitro.
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