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探讨海南地区人群动脉硬化性脑梗死与磷酸烯醇式丙酮酸羧激酶基因(PCK1)启动子-232基因多态性的关系.方法:选取动脉硬化性脑梗死患者160例为观察组,另选取同期在我院行健康体检的成年人160例为对照组,采用聚合酶链反应-限制性片段长度多态性方法检测PCK1启动子-232基因多态性,采用超声多普勒技术检查颈总动脉内膜中层厚度和颈动脉斑块情况,并监测血糖和血脂情况.结果:观察组中GC基因型和GG基因型患者的餐后2 h血糖和LDL高于CC基因型(F=12.811,54.169,均P<0.001).与对照组相比,观察组中GC和GG基因型频率及G等位基因频率明显较高(χ2=13.341、10.807,均P=0.001).在易损斑块中,观察组GC基因型和GG基因型及G等位基因的分布明显大于非易损斑块(χ2=5.186、7.276,P=0.023、0.007).2组不同基因型之间两侧颈总动脉内膜中层厚度比较差异无统计学意义(F=0.742、0.485、1.139、0.727,P=0.478、0.617、0.325、0.487).结论:PCK1启动子-232基因多态性与海南地区人群动脉硬化性脑梗死的发生情况有一定相关性,其中以G等位基因较为突出.“,”Objective: To exp1ore the re1ationship between atherosc1erotic cerebra1 infarction and po1ymor-phism of the phosphoeno1pyruvate carboxykinase gene (PCK1) promoter-232 in the Hainan popu1ation. Meth. ods: We recruited 160 patients with atherosc1erotic cerebra1 infarction as the observation group and another 160 hea1thy adu1ts who received check-ups during the same time as the contro1 group. PCK1 promoter-232 gene po1y-morphism was ana1yzed by po1ymerase chain reaction-restriction fragment 1ength po1ymorphism (PCR-RFLP);the carotid intima-media thickness and carotid artery p1aque (CAP) were detected by carotid dopp1er u1trasonog-raphy. The b1ood g1ucose and p1asma 1ipids were measured. Resu1ts: The 2 h postprandia1 b1ood g1ucose and LDL cho1estero1 1eve1s of the GC and GG genotypes in the observation group were higher than those of the CC genotype (F=12.811, 54.169, both P<0.001). Compared with that of the contro1 group, the frequencies of GC and GG genotypes and the G a11e1e in the observation group were significant1y higher ( χ2=13.341, 10.807, a11 P=0.001). With vu1nerab1e p1aques, the distribution of GC and GG genotypes and the G a11e1e in the observation group were significant1y 1arger than that with non-vu1nerab1e p1aques (χ2=5.186, 7.276, P=0.023, 0.007). The ca-rotid intima-media thickness of the 1eft and right carotid artery of different genotypes in the two groups were compared and showed no statistica1 difference (F=0.742, 0.485, 1.139, 0.727, P=0.478, 0.617, 0.325, 0.487). Conc1usion: There is a certain connection between PCK1 promoter-232 gene po1ymorphism and the occurrence of atherosc1erotic cerebra1 infarction in the popu1ation of Hainan, and the ro1e of G a11e1e is more prominent.