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目的 探讨转化生长因子(TGF)- β1基因修饰的树突状细胞(DC)对实验性自身免疫性重症肌无力(EAMG)大鼠病情的影响。方法 将8周龄的近交系、健康、雌性Lewis大鼠30只应用随机数字表分为6组:正常组、EAMG组、DC治疗组、pcDNA3 - TGF -β1- DC治疗组、pcDNA3 -DC治疗组和生理盐水对照组。除正常组外,其余均采用乙酰胆碱受体(AChR)蛋白2次免疫的方法诱导EAMG。初次免疫后第5天上述各组除正常组与EAMG组外分别皮下注射2×106 的DC、pcDNA3 - TGF- β1- DC、pcDNA3 - DC及等体积的生理盐水。观察各组大鼠的临床表现,并于初次免疫后7周进行低频重复电刺激,血清AChR抗体滴度检测及神经肌肉接头(NMJ)处的超微病理学观察。结果(1)免疫后1周左右,除正常组外各组均有1 ~2只大鼠出现轻度的肌无力症状,持续2 ~5d自行缓解。免疫后5周左右EAMG组、DC治疗组、pcDNA3 - DC治疗组及生理盐水对照组大鼠均相继出现肌无力症状,而pcDNA3 - TGF -β1 -DC治疗组仅1只四肢力量稍差。正常组大鼠始终未见异常。( 2 )EAMG组、DC治疗组、pcDNA3 - DC治疗组及生理盐水对照组大鼠低频电刺激肌电图波幅均有不同程度的衰减,而正常组及pcDNA3 - TGF- β1 DC治疗组(分别为3 .20±3. 70和5 .60±2 .70 )均无明显衰减;pcDNA3 - TGF -β1-DC治
Objective To investigate the effects of transforming growth factor (TGF) -1 gene modified dendritic cells (DCs) on the pathogenesis of experimental autoimmune myasthenia gravis (EAMG) in rats. Methods 30 healthy female Lewis rats aged 8 weeks were randomly divided into 6 groups: normal group, EAMG group, DC treated group, pcDNA3 - TGF-β1-DC treated group, pcDNA3-DC Treatment group and saline control group. Except for the normal group, EACG was induced by twice immunization with AChR protein. On the 5th day after the primary immunization, the above groups were subcutaneously injected with 2 × 106 DCs, pcDNA3 - TGF - β1 - DCs, pcDNA3 - DCs and equal volumes of normal saline, respectively, except the normal group and the EAMG group. The clinical manifestations of the rats in each group were observed. Low-frequency repetitive electrical stimulation, serum AChR antibody titers and ultrastructural observation at the neuromuscular junction (NMJ) were performed 7 weeks after the first immunization. Results (1) About 1 week after immunization, except for the normal group, 1 ~ 2 rats in each group showed mild symptoms of myasthenia gravis and continued to relieve themselves for 2 ~ 5 days. About 5 weeks after immunization, the mice in EAMG group, DC group, pcDNA3 - DC group and saline control group all developed muscle weakness symptoms one after the other, while the only one limbs in pcDNA3 - TGF-β1-DC group were less powerful. Normal rats have no abnormalities. (2) The amplitude of EMG of EAMG group, DC group, pcDNA3 - DC group and saline control group all attenuated to different extents, while the normal group and pcDNA3 - TGF - β1 DC treatment group Was 3.20 ± 3.70 and 5.60 ± 2.70), no significant attenuation; pcDNA3 - TGF-β1-DC