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目的比较玻璃化法与慢速程序法对人第3天(D3)胚胎冷冻复苏的效果。方法将接受体外受精-胚胎移植患者的取卵周期移植后剩余的D3胚胎分别进行玻璃化法或慢速程序法冷冻,比较胚胎解冻后的复苏率、妊娠率等指标。结果玻璃化法冷冻解冻78个周期,228个胚胎,存活210个(92.11%),完整胚胎172个(81.90%),移植77个周期,临床妊娠32个周期(41.56%),种植率20.95%;慢速程序法冷冻解冻157周期,702个胚胎,存活405个(57.69%),完整胚胎207个(51.11%),移植148周期,临床妊娠58个周期(39.19%),种植率17.78%。玻璃化法的胚胎复苏率、完整胚胎率高于慢速程序法(P<0.05);玻璃化法的临床妊娠率和种植率略高于慢速程序法,但差异无统计学意义。结论玻璃化法的冷冻复苏率比慢速程序法高,更适用于人D3胚胎的冷冻保存。
Objective To compare the effects of vitrification and slow procedure on embryonic day 3 (D3) freezing and resuscitation. Methods Remaining D3 embryos after oocyte retrieval in patients undergoing in vitro fertilization and embryo transfer were respectively frozen by vitrification or slow procedure, and the recovery rate and pregnancy rate after embryo thawing were compared. RESULTS: The vitrification method thawed 78 cycles, 228 embryos, 210 survived (92.11%), 172 intact embryos (81.90%), 77 cycles of transplantation, 32 clinical cycles of pregnancy (41.56%) and 20.95% 157 cycles (57.69%), 207 intact embryos (51.11%), 148 cycles of transplantation, 58 cycles of clinical pregnancy (39.19%) and 17.78% of implantation rate. Vitrification embryos recovery rate, complete embryo rate was higher than the slow method (P <0.05); vitiligo clinical pregnancy rate and planting rate slightly higher than the slow procedure, but the difference was not statistically significant. Conclusion The vitrification method has a higher rate of freezing and thawing than the slow procedure method and is more suitable for cryopreservation of human D3 embryos.