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研究并探讨非特异性酯酶 (NSE)和乙酰胆碱酯酶 (ACh E)活力在不同品系淡色库蚊 (Culespipiens pallens)各发育阶段的变化 ,为应用生物化学方法检测蚊虫抗药性及科学合理地进行蚊虫化学防治提供依据。用微量滴定板法测定单个蚊虫 NSE和 ACh E活力 ,结果显示 ,敏感品系、DDVP抗性品系和残杀威抗性品系淡色库蚊 NSE活力水平在不同发育阶段有变化 , ～ 龄幼虫较低 , 龄幼虫、蛹和 3日龄雌成蚊较高 ,在各发育阶段均是 DDVP抗性品系 NSE活力最高 ,残杀威抗性品系次之 ,敏感品系最低 ,其中 龄幼虫、蛹和 3日龄雌成蚊阶段三个品系间 NSE活力差别更明显 ;三个品系淡色库蚊 ACh E活力在 ～ 龄幼虫和蛹期较低 ,3日龄雌成蚊较高 ,三个品系间 ACh E活力稍有差异 ,从高到低依次为残杀威抗性品系、DDVP抗性品系和敏感品系。测定蚊虫 NSE活力判断抗药性可选择 龄幼虫、蛹和 3日龄未吸血雌成蚊 ;测定蚊虫 ACh E不敏感性判断抗药性可选择 3日龄未吸血雌成蚊 ;蚊虫化学防治中应选择低龄幼虫阶段施药
To investigate the changes of non-specific esterase (NSE) and acetylcholinesterase (ACh E) activity in different developmental stages of Culex pipiens pallens. To detect the resistance of mosquitoes using biochemical methods and to conduct scientific and rational mosquito control Chemical control to provide the basis. The microtiter plate method was used to determine the activities of NSE and ACh E in individual mosquitoes. The results showed that the NSE activity of Culex pipiens pallens in susceptible, DDVP-resistant and prophase-resistant strains varied at different developmental stages. Larvae, pupae and 3-day-old female adult mosquitoes were the highest. At each developmental stage, DDVP-resistant strains had the highest NSE activity, followed by propoxur resistant strains, and the lowest susceptible strains. The larvae, pupae and 3-day- The ACh E activity of three strains of Culex pipiens pallens was lower at the ~ larvae and pupae stage, while that of the adult at 3 days was higher, while the activity of ACh E was slightly different among the three strains , Followed by destroying Viagra resistant strains, DDVP resistant strains and sensitive strains from high to low. Determination of insecticidal activity of NSE in mosquitoes Selectable instar larvae, pupae and 3-day-old non-asymptomatic females; Determination of insecticides ACh E insensitivity to determine resistance to 3-day-old non-blood-sucking female adult mosquitoes; chemical control of mosquitoes should be selected Young larva stage application