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目的 :探讨caspase 3基因在缺血神经细胞死亡机制中的作用。方法 :采用大鼠大脑中动脉阻塞再灌流模型 (MCAO R) ,运用RT PCR和原位杂交技术观察缺血再灌流后caspase 3mRNA表达的时空分布动态变化 ,结合TUNEL技术观察其与凋亡的关系。结果 :脑缺血 3 0min再灌流 6h和缺血 2h再灌流 1h ,caspase 3mRNA在梗死边缘区的内侧诱导表达 ,并随着缺血时间或再灌流时间的延长而增强。caspase 3基因的表达与凋亡细胞的时空动态变化趋势基本一致。结论 :脑缺血损伤诱导caspase 3基因表达增强 ,caspase 3在介导神经细胞凋亡和缺血性脑损害中起关键作用
Objective: To investigate the role of caspase 3 in the mechanism of ischemic neuronal cell death. Methods: Rat model of middle cerebral artery occlusion and reperfusion (MCAO R) was established. The temporal and spatial distribution of caspase 3 mRNA expression after ischemia / reperfusion was observed by RT PCR and in situ hybridization. The relationship between the caspase 3 mRNA and apoptosis was observed by TUNEL technique . Results: The caspase 3 mRNA was induced in the medial border of the infarct zone 6 min after reperfusion at 30 min after cerebral ischemia and 1 h after reperfusion at 2 h after ischemia, and increased with the time of ischemia or the reperfusion time. The expression of caspase 3 was in consistent with the spatio-temporal dynamics of apoptotic cells. CONCLUSION: The expression of caspase 3 increases after cerebral ischemic injury and caspase 3 plays a key role in the mediation of neuronal apoptosis and ischemic brain damage