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目的研究阿司匹林对高脂喂养Wistar大鼠胰岛素敏感性和线粒体功能的影响。方法 24只Wistar大鼠,♂,分为对照组(NC)、高脂组(HF)和阿司匹林组(AF)(n=8),喂养8周后,以高胰岛素-正常葡萄糖钳夹测定大鼠胰岛素敏感性,取空腹血清测定大鼠谷草转氨酶(ALT)、谷丙转氨酶(AST)、高密度脂蛋白(HDL-C)、甘油三酯(TG)、空腹血糖(FBS),空腹胰岛素(FINS)的水平;将大鼠肝脏组织进行固定、包埋、切片和HE染色,观察肝脏组织学变化;取肝脏组织,测定肝糖原含量;提取肝细胞线粒体并分离线粒体超氧化物歧化酶,测定线粒体超氧化物歧化酶活性(SOD),以及提取肝组织总RNA,应用RT-PCR测定肝脏组织Mfn2 m RNA的表达。结果与NC组大鼠比较,HF组大鼠肝脏指数、TG、FBS、ALT、AST、INS明显升高(P<0.05),GIR和SOD显著降低(P<0.05),肝脏组织Mfn2 mRNA表达显著降低,肝细胞体积增大,胞质中有脂滴空泡;AF组大鼠上述各指标差异无统计学意义,显微结构无显著变化。结论阿司匹林可以改善高脂诱导的胰岛素抵抗及抑制脂肪肝的形成,这一作用可能是部分通过促进肝细胞Mfn2表达、改善线粒体功能实现的。
Objective To investigate the effect of aspirin on insulin sensitivity and mitochondrial function in high fat diet Wistar rats. Methods Twenty-four Wistar rats were divided into control group (NC), high fat group (HF) and aspirin group (n = 8), fed with high insulin-normal glucose clamp Insulin sensitivity and fasting serum were measured. The levels of ALT, AST, HDL-C, triglyceride, fasting blood glucose (FBS) and fasting insulin FINS). The liver tissues of rats were fixed, embedded, sliced and stained with hematoxylin and eosin (HE), and the histological changes of liver were observed. Liver tissues were taken for determination of hepatic glycogen content. Mitochondria and mitochondrial superoxide dismutase The activity of mitochondrial superoxide dismutase (SOD) was measured, and the total RNA of liver tissue was extracted. The expression of Mfn2 mRNA in liver tissue was detected by RT-PCR. Results Compared with NC group, liver index, TG, FBS, ALT, AST, INS in HF group were significantly increased (P <0.05), GIR and SOD were significantly decreased Decreased, the volume of hepatocytes increased, lipid droplets vacuoles in the cytoplasm. There was no significant difference in all the above indexes between AF group and the microstructure. Conclusion Aspirin can improve the insulin resistance induced by high fat and inhibit the formation of fatty liver. This effect may be partly through the promotion of Mfn2 expression in hepatocytes and the improvement of mitochondrial function.