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目的观察大鼠动脉粥样硬化病变过程中血管壁亲环素A(Cy PA)、Krüppel样转录因子2(KLF2)、内皮型一氧化氮合酶(e NOS)的表达变化。方法将雄性Wistar大鼠32只按数字表法随机分为对照组及动脉粥样硬化8、12、15周组,每组8只。给予动脉粥样硬化组高脂饲料喂养,并给予维生素D3注射液60万IU/kg一次性腹腔注射;给予对照组大鼠基础饲料喂养。在喂养第8、12、15周时处死大鼠,分离腹主动脉,行苏木素-伊红染色,免疫组化法检测动脉壁CyPA、KLF2、eNOS的表达水平。结果随着动脉粥样硬化病变进展,对照组以及动脉粥样硬化8、12、15周组大鼠分别呈现出血管正常结构、内皮细胞破坏、平滑肌细胞增生、粥样斑块形成以及钙化斑块形成等不同的特征。对照组与动脉粥样硬化8、12、15周组间血管壁CyPA、KLF2、eNOS的表达水平差异均有统计学意义(CyPA:0.0037±0.0018、0.0276±0.0090、0.0526±0.0129、0.1080±0.0388;KLF2:0.0932±0.0331、0.0415±0.0076、0.0207±0.0059、0.0014±0.0003;eNOS:0.0358±0.0185、0.0148±0.0080、0.0049±0.0025、0.0037±0.0026;F值分别为36.395、42.108、21.255,均P<0.05),且组间CyPA的表达呈增高趋势,组间两两比较差异有统计学意义(均P<0.05);KLF2表达水平呈进行性下降趋势,组间两两比较差异有统计学意义(均P<0.05);eNOS表达动脉粥样硬化12周组与15周组差异无统计学意义,其他组间差异均有统计学意义(均P<0.05)。结论伴随动脉粥样硬化病变进展,CyPA的表达进行性增高,KLF2、eNOS表达进行性下降。CyPA可能是致动脉粥样硬化病变的途径之一。
Objective To observe the expression changes of CyPA, Krüppel-like transcription factor 2 (KLF2) and eNOS during atherosclerotic lesions in rats. Methods 32 male Wistar rats were randomly divided into control group and atherosclerotic groups of 8, 12 and 15 weeks according to the digital table method, with 8 in each group. Given atherosclerosis group of high-fat diet, and given vitamin D3 injection of 60 million IU / kg intraperitoneal injection; control group rats fed on the basis of feed. Rats were sacrificed at the 8th, 12th and 15th week after feeding. The abdominal aorta was isolated and stained with hematoxylin-eosin. The expression of CyPA, KLF2 and eNOS in the arterial wall was detected by immunohistochemistry. Results With the development of atherosclerosis, the rats in the control group, at 8, 12, and 15 weeks of atherosclerosis showed normal vascular structure, endothelial cell destruction, smooth muscle cell proliferation, plaque formation and calcified plaque The formation of such different characteristics. The expression levels of CyPA, KLF2 and eNOS in vessel wall of control group and atherosclerotic group at 8, 12 and 15 weeks were significantly different (CyPA: 0.0037 ± 0.0018,0.0276 ± 0.0090,0.0526 ± 0.0129,0.1080 ± 0.0388; The values of FN were 36.395, 42.108 and 21.255, respectively, P <0.05 (P <0.05) ), And the expression of CyPA between the groups showed an increasing tendency. There was a significant difference between the two groups (P <0.05), while the expression of KLF2 decreased progressively. The difference between the two groups was statistically significant (both P <0.05). There was no significant difference in the expression of eNOS between atherosclerotic lesions of 12 weeks and 15 weeks. There were significant differences among the other groups (all P <0.05). Conclusions With the development of atherosclerotic lesions, the expression of CyPA increases progressively and the expressions of KLF2 and eNOS decrease progressively. CyPA may be one of the pathways leading to atherosclerotic lesions.