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用共振瑞利散射(resonance Rayleigh scattering,RRS)光谱和紫外吸收光谱及荧光光谱研究了博莱霉素(bleomycinA2,BLMA2)-Cu(Ⅱ)配合物的形成及其与DNA的反应.在水溶液中,BLMA2和Cu(Ⅱ)能形成1:1的配合物,产生新的吸收光谱,并能观察到BLMA2的荧光猝灭.该配合物能与DNA作用生成三元离子缔合物,引起RRS的显著增强.讨论了BLMA2-Cu(Ⅱ)配合物的形成条件及影响因素;对该配合物与DNA的结合模式和反应机理进行了讨论;探讨了博莱霉素荧光猝灭的类型,并建立了一种用BLMA2-Cu(Ⅱ)配合物作探针RRS法测定DNA的高灵敏度、简单、快捷的分析方法.该方法的检出限(3σ)分别为7.2ng·mL-1(ctDNA)、7.1ng·mL-1(sDNA)、18ng·mL-1(hsDNA),能用于痕量DNA的测定.
The formation of bleomycin (BLMA2) -Cu (Ⅱ) complex and its reaction with DNA were studied by resonance Rayleigh scattering (RRS) spectroscopy, UV absorption spectroscopy and fluorescence spectroscopy. , BLMA2 and Cu (Ⅱ) can form a 1: 1 complex to generate a new absorption spectrum, and the fluorescence quenching of BLMA2 can be observed. This complex can interact with DNA to generate ternary ion-association complexes that cause RRS The formation conditions and influencing factors of BLMA2-Cu (Ⅱ) complex were discussed. The binding mode and reaction mechanism of the complex with DNA were discussed. The type of fluorescence quenching of bleomycin was discussed and established A highly sensitive, simple and rapid method for the determination of DNA with BLMA2-Cu (Ⅱ) complex as probe RRS was developed.The detection limits (3σ) were 7.2 ng · mL-1 (ctDNA) , 7.1ng · mL-1 (sDNA), 18ng · mL-1 (hsDNA), which can be used for the determination of trace DNA.