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采用生长速率法,以相对抑菌率、有效中浓度(EC50)值为指标,测定了壳聚糖、硝酸锌、山梨酸钾以及它们复配于膨润土时,对芒果蒂腐和芒果炭疽病菌的抑制效果。结果表明:在病原菌离体平板培养试验中,蒂腐与炭疽菌分别于28℃的恒温培养箱中培养2、4 d,通过观察菌丝生长情况发现,上述材料对2种芒果病原菌菌丝的生长均有良好抑制效果,且随着抑菌剂浓度的增加其抑制效果增强;其中以壳聚糖的抑菌活性最为显著,EC50值分别为2.0188、1.1288 g/L。与3种抑菌剂复配的膨润土基材料对芒果病原菌有一定的抑菌效果,但抑菌效果略低于单一使用的抑菌剂。
The growth rate method was used to determine the relative inhibitory rate and EC50 value of chitosan, zinc nitrate, potassium sorbate and their compounds in bentonite, Inhibitory effect. The results showed that during the in vitro culture of pathogen, the decoctions and anthracnose were cultured in constant temperature incubator at 28 ℃ for 2 and 4 days, respectively. By observing the growth of mycelium, The inhibitory effect was enhanced with the increasing concentration of bacteriostat. Among them, the antibacterial activity of chitosan was the most significant, with EC50 values of 2.0188 and 1.1288 g / L, respectively. The bentonite-based materials compounded with three kinds of bacteriostats had some bacteriostatic effects on mango pathogens, but the bacteriostatic effect was slightly lower than that of the single used bacteriostats.