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目的:针对糖化血红蛋白(GHb)常用检测方法进行比较、评估,为临床应用提供参考依据。方法:实验对象来自于该院内科及体检中心的被检者,其中正常对照组56例,观察组58例,分别采用层析法、电泳法、HPLC法和免疫法方法对血液样本进行检测,测定Hb A1c的浮动值并收集数据进行分析。结果:除层析法外,HPLC法(1.46%~1.18%)、免疫法(2.25%~2.28%)和电泳法(1.66%~1.42%)的组间和组内精密度均小于5%;加入各种干扰因素后,这三种方法得到的测得的Hb A1c浮动小于<0.2%。结论:经临床对照实验可见,除层析法外其他三种方法均符合临床检测要求,其中HPLC检测方法在糖化血红蛋白值检测中的稳定性、可靠度相对优越,在临床应用时可根据实际情况合理选用检测方法。
Objective: To compare and evaluate the commonly used detection methods of glycated hemoglobin (GHb), and provide a reference for clinical application. Methods: The subjects were from the medical and physical examination center of the hospital were seized, of which 56 cases of normal control group and observation group of 58 cases, respectively, by chromatography, electrophoresis, HPLC and immunological methods of blood samples were detected, The floating value of Hb A1c was measured and the data collected for analysis. Results: In addition to chromatography, the intra- and intra-assay precision were less than 5% by HPLC (1.46% -1.18%), immunoassay (2.25% -2.28%) and electrophoresis (1.66% -1.42% The measured Hb A1c fluctuations obtained by these three methods were less than <0.2% after adding various disturbing factors. Conclusion: The clinical control experiments show that, in addition to chromatography other than the three methods are in line with clinical testing requirements, including HPLC detection of HbA1c in the stability of the test, the reliability is relatively superior in clinical application can be based on the actual situation Reasonable selection of detection methods.