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目的:建立不同产地大黄的分子系统学基础,为大黄的基源鉴定和品质评价提供分子依据。方法:采用PCR技术获得ITS基因,进行测序,经软件进行统计分析,计算各样本间的遗传距离并建立系统树。结果:大黄ITS序列G+C含量较高,达66.55%～68.49%,8个样品ITS(包括5.8s)序列的长度范围为562～568。所有样品5.8srDNA高度一致,除DH5样品有两个位点的碱基转换外,其余无碱基差异。结论:ITS序列特征可以作为大黄种间鉴别的有效分子标记,药用大黄在属内与其它种关系较远。
OBJECTIVE: To establish the molecular systematic basis of rhubarb in different habitats and to provide the molecular basis for identification and quality evaluation of rhubarb. Methods: The ITS gene was obtained by PCR and sequenced. The software was used for statistical analysis. The genetic distance between samples was calculated and the phylogenetic tree was constructed. Results: The content of G + C in rhubarb ITS sequence was high (66.55% -68.49%), and the sequence length of ITS (including 5.8s) in 8 samples ranged from 562- 568. All samples 5.8srDNA highly consistent, except for the DH5 sample has two positions of the base conversion, the rest no base difference. CONCLUSION: ITS sequence can be used as an effective molecular marker for the identification of Rhizobium species. Medicinal rhubarb has a long relationship with other species in genus.