提出以次氯酸钠－芦丁－盐酸氨基脲化学发光新体系，结合流动注射技术建立了测定芦丁的化学发光新方法。芦丁质量浓度在４．０×１０－１１～１．０×１０－８ｇ／Ｌ范围内与发光信号呈线性关系。检出限为１．３×１０－１１ｇ／Ｌ。相对标准偏差为２．６％（ｎ＝１１；ｃｓ＝６×１０－１０ｇ／Ｌ） A new chemiluminescence method for the determination of rutin with sodium hypochlorite-rutin-hydrochloric acid semicarbazide chemiluminescence system was proposed and a flow injection technique was established. The concentration of rutin in the range of 4.0 × 10-11 ~ 1.0 × 10-8g / L showed a linear relationship with the luminescence signal. The detection limit was 1.3 × 10-11g / L. The relative standard deviation was 2.6% (n = 11; cs = 6 × 10-10g / L)