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目的:建立益气软皮丸中毛蕊异黄酮葡萄糖苷的含量测定方法。方法:采用高效液相色谱法,选择Diamonsil TC-C18色谱柱(4.6mm×250mm,5μm),以乙腈-0.2%甲酸溶液为流动相进行梯度洗脱,流速为1.0mL·min-1,柱温为30℃,检测波长为260nm。结果:毛蕊异黄酮葡萄糖苷在106~636ng质量浓度范围内与峰面积积分呈良好线性关系(r=0.999 6,n=6),平均回收率为97.48%,RSD为0.82%(n=6)。结论:该法简便、准确、重现性好,可用于益气软皮丸制剂中毛蕊异黄酮葡萄糖苷的含量测定。
Objective: To establish a method for determination of calycosin glucoside in Yiqi Ruanpi Wan. METHODS: Diamonsil TC-C18 column (4.6 mm × 250 mm, 5 μm) was selected by HPLC. The mobile phase consisted of acetonitrile-0.2% formic acid as mobile phase with gradient elution at a flow rate of 1.0 mL · min -1. Temperature is 30 ℃, detection wavelength is 260nm. Results: There was a good linear relationship between the concentration of calycosin and peak area in the range of 106 ~ 636ng (r = 0.999 6, n = 6). The average recovery was 97.48% and the RSD was 0.82% (n = 6). Conclusion: The method is simple, accurate and reproducible. It can be used to determine the content of calycosin glucoside in Yiqi Ruanpi Wan preparations.