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采用高效液相色谱法测定了血清中海洛因的代谢物。本法使用YWG -C1 8分析柱 ,以甲醇 -水 ( 3 :7,含 0 5%三乙胺 ,用冰醋酸调节pH值 =6 8)为流动相 ,流速 1mL/min ,检测波长2 54nm。内标物为苯巴比妥 ;对照品吗啡的线性范围为 0 1 2～ 1 92 μg/mL ,相关系数为 0 9990 ;6-单乙酰吗啡的线性范围为 0 1 8～ 1 62 μg/mL ,相关系数为 0 9989。本法简便 ,灵敏 ,适用于吸毒者血中海洛因含量的监测。
Metabolites of heroin in serum were determined by high performance liquid chromatography. This method uses YWG-C1 8 analytical column with methanol-water (3: 7, containing 0 5% triethylamine, pH adjusted with glacial acetic acid = 6 8) as the mobile phase at a flow rate of 1 mL / min and a detection wavelength of 254 nm . The internal standard was phenobarbital. The standard range of morphine was 0 1 2 ~ 1 92 μg / mL with a correlation coefficient of 0 9990. The linear range of 6-monoacetylmorphine was 0 1 8 ~ 1 62 μg / mL , The correlation coefficient is 0 9989. This method is simple, sensitive and suitable for the monitoring of heroin content in the blood of drug addicts.