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背景:糖尿病晚期并发症的发生与遗传因素有关。有报道血管紧张素Ⅰ转换酶(angiotensinⅠ-convertingenzyme,ACE)基因是糖尿病心血管并发症发生的风险因子。目的:探讨ACE基因多态性与2型糖尿病心血管并发症之间的相关性。设计:以2型糖尿病患者为研究对象的病例-对照研究。单位:一所医学院的生物科学系普通生物学教研室。对象:本研究于2001-02/2002-06在华北煤炭医学院分子生物学实验室完成。研究对象均为本医学院附属医院门诊及住院2型糖尿病患者,根据是否合并心肌梗死分为两组。心肌梗死患者组68例,其中男29例,女39例,年龄(65±3)岁;非心肌梗死组190例,其中男161例,女29例,年龄(60±1)岁。纳入标准:均符合1985年WHO2型糖尿病诊断标准,心肌梗死诊断根据MRI确诊;排除标准:资料不完整或不合作者。方法:按常规酚/氯仿法提取基因组DNA,扩增产物用20mL/L琼脂糖凝胶电泳分析,溴化乙锭染色观察结果。对不同患者组等位基因和基因型频率进行比较。主要观察指标:两组患者ACE基因的等位基因和基因型分布比较。结果:2型糖尿病心肌梗死患者64.7%与非心肌梗死患者55.0%比()()较,DD纯合子频率显著增高(41.2%vs33.2%),差异具有显著性意义(χ2=0.833,P<0.05),D等位基因频率也显著增高,差异也具有显著性意义(χ2=3.8491,P<0
Background: The late complications of diabetes are related to genetic factors. It has been reported that the angiotensin-converting enzyme (ACE) gene is a risk factor for the development of cardiovascular complications of diabetes mellitus. Objective: To investigate the association between ACE gene polymorphism and cardiovascular complications of type 2 diabetes mellitus. Design: Case-control study of patients with type 2 diabetes. Unit: a Department of Biology, Faculty of Biology, Department of General Biology. PARTICIPANTS: This study was performed at the Molecular Biology Laboratory of North China Coal Medical College from February 2001 to June 2002. Subjects were all outpatients and inpatients with type 2 diabetes mellitus in this Affiliated Hospital of the Medical College. Patients were divided into two groups according to whether they had MI or not. Sixty-eight patients with myocardial infarction, including 29 males and 39 females, were aged (65 ± 3) years. In non-myocardial infarction group, there were 190 males and 161 females with 29 females, with an age of (60 ± 1) years. Inclusion criteria: all in line with the WHO WHO diagnostic criteria for type 2 diabetes in 1985, diagnosis of myocardial infarction diagnosed according to MRI; Exclusion criteria: incomplete or not data. Methods: Genomic DNA was extracted by conventional phenol / chloroform method. The amplified product was analyzed by 20mL / L agarose gel electrophoresis and ethidium bromide staining. The allele and genotype frequencies of different patient groups were compared. MAIN OUTCOME MEASURES: Comparison of allele and genotype distribution of ACE gene in two groups. RESULTS: The frequency of DD homozygote was significantly higher in 64.7% of patients with type 2 diabetes mellitus than in 55% of patients with non-myocardial infarction (41.2% vs 33.2%) (χ2 = 0.833, P <0.05), and the frequency of D allele was also significantly increased (χ2 = 3.8491, P <0