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目的观察川芎嗪对子痫前期样大鼠的治疗作用以及对胎盘组织中缺氧诱导因子(HIF)-1α表达水平的影响。方法雌性SD孕鼠随机分为对照组、子痫前期模型组和川芎嗪治疗组,川芎嗪设3个剂量组(10,20,40 mg·kg-1),每组8只。对照组外,第14 d用尾静脉缓慢滴注内毒素方法诱导大鼠子痫前期模型。治疗组于第14～21 d每天腹腔注射相应剂量川芎嗪,对照组和模型组注射等量生理盐水。分别于第10,15,19 d测量血压、24 h尿蛋白量;孕21 d行剖宫产手术,分离胎盘组织并称重;用荧光定量PCR方法检测胎盘组织中HIF-1α的表达水平。结果与对照组相比,模型组大鼠血压和24 h尿蛋白量在19 d时均显著升高,胎盘重量明显减轻,而胎盘组织中HIF-1α的表达水平显著升高。川芎嗪降低19 d时大鼠血压和24 h尿蛋白量,增加胎盘重量,并降低胎盘组织中HIF-1α的表达水平,与模型组相比有统计学意义。结论川芎嗪对子痫前期大鼠有治疗作用,其机制可能与抑制胎盘组织中的HIF-1α表达有关。
Objective To observe the therapeutic effect of ligustrazine on preeclampsia rats and its effect on the expression of hypoxia inducible factor (HIF) -1α in placenta. Methods Female SD pregnant rats were randomly divided into control group, preeclampsia model group and ligustrazine treatment group, ligustrazine three dose groups (10,20,40 mg · kg-1), 8 in each group. In the control group, the rat preeclampsia model was induced by slowly instillation of endotoxin on the 14th day. The treatment group was given intraperitoneal injection of ligustrazine on the 14th-21st day, and the control group and model group were injected with the same amount of saline. Blood pressure and 24-hour urinary protein were measured on the 10th, 15th and 19th day respectively. Cesarean section was performed on the 21st day of gestation and placental tissue was isolated and weighed. The expression of HIF-1α in the placenta was detected by fluorescence quantitative PCR. Results Compared with the control group, the blood pressure and 24 h urinary protein in model group increased significantly on day 19 and placental weight decreased significantly, while the expression of HIF-1α in placenta significantly increased. Ligustrazine reduced the blood pressure and 24 h urinary protein, increased the weight of placenta and decreased the expression of HIF-1α in placental tissue at 19 d, which was statistically significant compared with model group. Conclusion Tetramethylpyrazine has a therapeutic effect on preeclampsia rats, which may be related to the inhibition of HIF-1α expression in placenta.