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目的通过观察雌激素对去势雌鼠血清及膀胱e-NOS、AQP1含量的影响,进一步探讨雌激素替代治疗对绝经后尿道综合征的机制。方法成年健康SD雌性大鼠24只,随机分为空白组、对照组、尼尔雌醇组。摘除卵巢建立大鼠雌激素缺乏模型,灌胃给药4周后,用ELISA法测定血清及膀胱组织中e-NOS、AQP1的含量。结果尼尔雌醇组与对照组比较,血清e-NOS、AQP1含量显著增高(P<0.05);与空白组比较,差异无统计学意义(P>0.05)。去势后膀胱e-NOS明显下降,补充雌激素后恢复正常水平,而AQP1去势前、后无显著改变,补充雌激素后亦无明显改变趋势。结论卵巢切除导致大鼠膀胱e-NOS发生下降,补充雌激素后恢复,而AQP1无差异。e-NOS在绝经后尿道综合征中起到直接作用。
Objective To investigate the effect of estrogen on e-NOS and AQP1 levels in ovaries and ovaries in ovariectomized female rats and to explore the mechanism of estrogen replacement therapy for postmenopausal urethral syndrome. Methods Twenty-four adult SD female rats were randomly divided into blank group, control group and nilestriol group. The ovariectomized rat model of estrogen deficiency was established. After 4 weeks of intragastric administration, the levels of e-NOS and AQP1 in serum and bladder tissues were determined by ELISA. Results Compared with the control group, the levels of e-NOS and AQP1 in the nilestriol group were significantly increased (P <0.05). There was no significant difference between the nilestriol group and the control group (P> 0.05). Eosinophilic bladder eosinophil decreased significantly after estrogen replacement, returned to normal levels, and before and after castration of AQP1 no significant change, after estrogen replacement there was no significant change in the trend. Conclusion Ovariectomy results in the decrease of urinary e-NOS in rats. After ovariectomized, estrogen recovered, but there was no difference in AQP1. e-NOS plays a direct role in postmenopausal urethral syndrome.