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研究益气活血方药理血清对大鼠贮脂细胞增殖作用机制。通过制备 3周、5周、7周的肝纤维化模型大鼠血清、正常大鼠血清和益气活血复方药物血清 ,培养肝星形细胞HSC(Hepaticstellatecells)和肝Bel74 0 2瘤株 ,采用MTT法观察和分析HSC的增殖作用。结果表明 :①益气活血方剂血清能抑制 5周、7周模型血清培养的继代HSC的增殖 ,P <0 .0 5。②益气活血方剂血清可促进 3周模型血清培养的继代HSC和模型血清培养的原代HSC的增殖 ,P <0 .0 5。③益气活血方剂血清和3周模型血清共同作用促进培养的Bel74 0 2的增殖 ,P <0 .0 5。提示益气活血方剂能够对HSC和Bel74 0 2细胞的增殖产生影响。HSC的生活状态 ,即HSC发育时相是决定益气活血方剂具有抑制或促进增殖作用的关键事件
To study the mechanism of Yiqi Huoxue decoction pharmacological serum on the proliferation of rat fat-storing cells. Hepatic stellate cell HSC (Hepaticstellate cells) and hepatic Bel74 0 2 tumor strains were cultured by preparing hepatic fibrosis model rat serum, normal rat serum, and Yiqi Huoxue compound serum at 3, 5, and 7 weeks, using MTT. Observe and analyze the proliferation of HSCs. The results showed that: 1 Serum of Yiqi Huoxue Prescription inhibited the proliferation of subcultured HSC cultured at 5 weeks and 7 weeks, P<0.05. 2 Yiqi Huoxue Prescription Serum can promote the proliferation of primary HSC cultured in subcultured HSC and model serum cultured in 3 weeks model serum, P<0.05. 3 The combination of Yiqi Huoxue Prescription Serum and 3 weeks model serum promoted the proliferation of cultured Bel74 0 2 , P <0.05. Tip Yiqi Huoxue Formula can affect the proliferation of HSC and Bel74 02 cells. HSC’s state of life, HSC developmental phase, is a key event that determines whether Yiqi Huoxue Formula can inhibit or promote proliferation.