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用循环伏安法将电子媒介体亚甲蓝电聚合在铂电极表面上,使其表面形成均匀的聚亚甲蓝膜,通过磁铁对磁性纳米材料的吸附作用将磁性纳米CoFe2O4/SiO2吸附在聚亚甲蓝表面,接着先后吸附葡萄糖氧化酶和磁性纳米CoFe2O4/SiO2,最后用亚甲蓝包埋电极,从而制得葡萄糖氧化酶生物传感器。实验结果表明,该葡萄糖传感器的循环伏安图中氧化峰电流与葡萄糖的浓度在1.2×10-7～6.5×10-5 mol/L范围内呈良好线性关系,相关系数r=0.9962,检出限为6.2×10-8mol/L(S/N=3)。
Cyclic voltammetry was used to electropolymerize the electron mediator methylene blue on the surface of platinum electrode to form a uniform polymethylene blue film on the surface. Magnetic nanocomposite CoFe2O4 / SiO2 was adsorbed on the surface of poly Methylene blue surface, followed by adsorption of glucose oxidase and magnetic nano-CoFe2O4 / SiO2, and finally with methylene blue embedded electrode, thereby producing glucose oxidase biosensor. The experimental results show that the oxidation peak current of the glucose sensor has a good linear relationship with the concentration of glucose in the range of 1.2 × 10-7 ~ 6.5 × 10-5 mol / L, the correlation coefficient r = 0.9962, The limit is 6.2 × 10 -8 mol / L (S / N = 3).