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目的 了解中国南京地区有无TT病毒(TTV)感染。方法采用套式聚合酶链反应(PCR)检测病因未明的肝炎患者血清中的TTV-DNA,并对PCR扩增产物进行序列测定。结果 在14例非甲～戊型肝炎患者的血清中,共检出8例TTVDNA阳性者,对其中一株进行序列测定,该序列与日本TTV部分基因(CLON22)和TTV中国株1相对应位置的核苷酸同源性很高,分别达97.65％和98.99％。结论 在中国南京地区的肝炎患者中存在着TTV感染。
Objective To understand the presence or absence of TT virus (TTV) infection in Nanjing, China. Methods TTV-DNA in sera of hepatitis patients with unknown etiology was detected by nested polymerase chain reaction (PCR) and the PCR amplification products were sequenced. Results Totally 8 TTVDNA positive cases were detected in the serum of 14 patients with non-hepatitis E-hepatitis E virus, and one of them was sequenced. The sequence was in accordance with the CLON22 and TTV Chinese strain 1 Nucleotide homology was high, respectively 97.65% and 98.99%. Conclusion TTV infection exists in hepatitis patients in Nanjing, China.