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胆碱乙酰转移酶(ChAT)催化乙酰CoA和胆碱合成神经递质乙酰胆碱(ACh),ChAT广泛存在于脊椎动物和无脊椎动物,包括某些寄生蠕虫的神经组织中。本文首次报告了几种丝虫的特高水平ChAT,并比较了其酶活力的差异,分析了多种药物对ChAT活力的影响,旨在了解丝虫ChAT的生理功能,以及作为化疗靶子的可能性。分别制备虫体(马来丝虫、彭亨丝虫、棉鼠丝虫成虫和微丝蚴,班氏丝虫微丝蚴,美州钩虫和鼠鞭虫成虫)的组织匀浆,超声粉碎,离心后(12000×g,20分钟)上清待测。ChAT活力测定:参照Fonnum放射化学法,活力单位定为37℃下每分钟形成1μmol ACh,
ChAT catalyzes the synthesis of the acetylcholine (ACh), a neurotransmitter of acetyl-CoA and choline, which is found extensively in the nervous tissues of vertebrates and invertebrates, including some parasitic worms. In this paper, we report for the first time the ultra-high levels of ChAT of several filarial worms and compare their differences in enzyme activity. The effects of various drugs on ChAT activity were analyzed in order to understand the physiological function of ChAT and the potential as a chemotherapy target Sex. Tissue homogenates of adult worms (Malayan filaments, Penaeid silkworm, adult cotton budworm and microfilariae, filarial microflora of Bancroftian filaria, adult hookworm and rat whipworm) were prepared and sonicated and centrifuged respectively (12000 × g, 20 minutes) supernatant to be measured. ChAT activity assay: Referring to the Fonnum radiochemical method, the viability unit was set to 1 μmol of ACh per minute at 37 ° C,