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目的:探讨肾移植受者外周血中肿瘤坏死因子(TNF-α)基因多态性与急性排斥反应的关系。方法:应用序列特异性引物聚合酶链反应(PCR-SSP)测定62例肾移植受者外周血中TNF-α的基因型,并结合供受者HLA配型情况,比较各基因型对急性排斥反应发生率的影响。结果:在HLA-DR错配的情况下,TNF-α等位基因为高分泌型者,其术后急性排斥反应发生率较低分泌型者高(P<0.05)。结论:TNF-α基因型对肾移植排斥反应发生率有明显影响,可据此或可制定更为合理的个体化免疫抑制治疗方案。
Objective: To investigate the relationship between the polymorphism of tumor necrosis factor (TNF-α) gene and the acute rejection in peripheral blood of renal allograft recipients. Methods: The genotypes of TNF-α in peripheral blood from 62 renal transplant recipients were determined by PCR-SSP. The genotypes of TNF-α in peripheral blood of 62 renal transplant recipients were compared. Effect of reaction rate. Results: In the case of HLA-DR mismatch, the TNF-α allele was highly secreted, with a higher incidence of postoperative acute rejection (P <0.05). Conclusion: The genotype of TNF-α has a significant effect on the incidence of renal allograft rejection. Therefore, more reasonable individual immunosuppressive regimens may be formulated accordingly.