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用聚丙烯酰胺凝胶电泳法，研究接种植物内生性生防菌后，其诱导棉花抗黄萎病过程中棉花茎叶组织中的过氧化物酶、超氧化物歧化酶（ＳＯＤ）和酯酶活性的变化。①诱导接种植物内生性生防菌７３ａ后，接种点附近棉茎中的过氧化物酶活性高，表现为酶带宽，颜色深，活性明显强于单用针剌的对照，而针剌对照又明显高于空白对照；接种后４天，接种７３ａ等内生性生防菌的酶谱比针剌对照及空白对照多１条Ｒｆ为０．２８的酶带。挑战接种后１０天测定，接种点附近的棉茎间过氧化物酶活性同对照已无明显差异。棉花叶片组织测定表明，诱导接种４天后，接种内生菌７３ａ的叶片过氧化物酶活性强于针剌对照。②诱导接种７３ａ后２天，接种点附近的棉茎中ＳＯＤ酶谱有３条酶带，多于空白对照的２条，且活性强。③诱导接种７３ａ后２４小时，接种点附近棉茎组织酯酶酶谱比空白对照多１条Ｒｆ为０．６５的宽酶带
Polyacrylamide gel electrophoresis was used to study the effects of endophytic biocontrol inoculated plants on the activities of peroxidase, superoxide dismutase (SOD) and esterase Changes in activity. ① Induced endophytic biocontrol fungi 73a inoculation plant, the peroxidase activity in the cotton stem near the inoculation point is high, showing the enzyme bandwidth, dark color, activity was significantly stronger than the single needle puncture control, and acupuncture contrast Significantly higher than the blank control; 4 days after inoculation 73a and other endogenous biocontrol bacteria than the acupuncture needle control and blank control more than 1 Rf 0.28 enzyme band. Ten days after inoculation challenge, there was no significant difference in the activity of the peroxidase between cotton buds in the vicinity of the inoculation point with the control. The results of cotton leaf tissue test showed that after inoculation for 4 days, the peroxidase activity of endophyte 73a inoculation was stronger than acupuncture control. ② Two days after inducing 73a inoculation, SOD bands in the cotton stem near the inoculation site had 3 bands, more than 2 in the blank control group, and had strong activity. ③At 24 hours after the inoculation of 73a, the esterase zymogram in the cotton stem tissue near the inoculation point was more than that of the blank control by one more wide band with Rf 0.65