【摘 要】
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Objective: The aim of the study was to examine the effect of Sp1 on the expression of the human telomerase reverse transcriptase (hTERT) gene in human colorecta
【机 构】
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Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China;
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Objective: The aim of the study was to examine the effect of Sp1 on the expression of the human telomerase reverse transcriptase (hTERT) gene in human colorectal carcinoma SW480 cells. Methods: The Sp1 shRNA plasmid was transfected into colorectal carcinoma SW480 cells line by liposome mediation for transient expression. After Sp1 shRNA plasmid transfected SW480 cells, the exogenous Sp1 protein expression was determined by the method of Weste blot. At same time, hTERT mRNA expression was detected by RT-PCR, telomerase activity was determined by the telomeric repeat ampli.cation protocol (TRAP) assay, and the apoptotic rate of cells was also tested by flow cytometry. Results: The protein expressions of Sp1 gene could be reduce by transfecting of pGenesil-1-Sp1(+) recombinant plasmid into SW480 cells. The apoptotic rate was increased compared with pGenesil-1-Sp1(–)/SW480 and SW480 (P < 0.05), which indicated that low-expression of Sp1 gene could lead to low level of telomerase activity and induce apoptosis. Conclusion: Silencing Sp1 may suppress the activity of telomerase by inhabiting hTERT gene expression.
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