目的:研究光动力疗法(photodynamic therapy，PDT)和选择性环氧合酶-2(cyclooxygenase-2，COX-2)抑制剂NS-398联合对人胆管癌细胞QBC939的抑制作用。方法:体外培养人胆管癌QBC939细胞株，分别设立PDT处理组、NS-398浓度组、二者联合组和空白对照组，将血卟啉衍生物(HPD)浓度(0、2、4、6、8、10 μg/ml)，光照强度(0、5、10、15 J/cmn 2)和NS-398浓度(0、25、50、100、200 μmol/L)分别联合作用；利用CCK8法检测各组处理对QBC939细胞生长的相对抑制率；采用流式细胞实验检测QBC939细胞的凋亡情况；采用Transwell法检测QBC939细胞的体外侵袭情况。n 结果:PDT和NS-398在体外均能单独抑制QBC939细胞生长，HPD浓度8 μg/ml，光照强度5 J/cmn 2联合50 μmol/L的NS-398作用后，细胞生长抑制率达95%，二者联合组与PDT处理组、NS-398浓度组和空白对照组比较均有统计学差异(n P<0.05)，继续增加二者的强度，细胞生长抑制率变化不明显；流式细胞实验表明PDT和NS-398联合作用能够明显促进QBC939细胞的早期凋亡，二者联合组的细胞凋亡率为(66.18±1.22)%，空白对照组、PDT处理组和NS-398浓度组的细胞凋亡率分别为(4.16±1.25)%、(22.19±1.17)%、(23.21±1.65)%，二者联合组与其它三组相比差异均有统计学意义(n P<0.05)；HPD浓度4 μg/ml，光照强度5 J/cmn 2联合25 μmol/L的NS-398作用后，QBC939细胞生长无明显抑制，但其体外侵袭能力降低，二者联合组的侵袭细胞数(8.16±1.65)少于空白对照组(31.22±1.25)、PDT处理组(18.09±1.17)和NS-398浓度组(19.21±1.72)，差异具有统计学意义(n P<0.05)。n 结论:PDT和NS-398联合作用可以促进QBC939细胞早期凋亡，抑制其侵袭能力，进而起到对QBC939细胞生长的抑制作用。“,”Objective:To study the effect and the mechanism of photodynamic therapy(PDT) combined with NS-398 on cholangiocarcinoma QBC939 cell lines.Methods:In vitro, QBC939 cells were divided into PDT treatment group, NS-398 concentration group, combined group and blank control group, which were exposed to 0, 2, 4, 6, 8, 10 μg/ml Hematoporphyrin derivative (HPD) with 0, 5, 10, 15 J/cm n 2 light radiation and 0, 25, 50, 100, 200 μmol/L NS-398 respectively. The growth of QBC939 cells was determined by CCK8 assay and the growth inhibition ratio was calculated. Flow cytometry and transwell migration assays were used to detect apoptosis and invasion of QBC939 cells respectively.n Results:PDT combined with NS-398 could significantly suppress the growth of QBC939 cells in vitro(n P<0.05). When the concentration of HPD was 8 μg/ml, the light radiation was 5 J/cmn 2 and NS-398 was 50 μmol/L, the relative growth inhibition rate of QBC939 cells was about 95%. If the intensity of PDT and NS-398 were increased, no statistical differences were detected. It’s indicated that PDT combined with NS-398 could promote QBC939 cells apoptosis at the early stage through the flow cytometry detection. The rate of apoptosis of QBC939 cells was (66.18±1.22)% in combined group, the apoptotic rate of blank control group, PDT treatment group and NS-398 concentration group were (4.16±1.25)%, (22.19±1.17)%, (23.21±1.65)% respectively, which differences were statistically significant( n P<0.05). When the concentration of HPD was 4 μg/ml, the light radiation was 5 J/cmn 2 and NS-398 was 25 μmol/L, it had no obviously inhibitory effect on QBC939 cells growth, but could obviously inhibit cells invasion through transwell migration assays. The number of invasive cells in combined group (8.16±1.65) was significantly lower than that in blank control group (31.22±1.25), PDT treatment group (18.09±1.17) and NS-398 concentration group (19.21±1.72), and significant differences were observed between the combined group and the other three groups ( n P<0.05).n Conclusion:PDT combined with NS-398 could promote apoptosis and inhibit invasion of QBC939 cells in vitro, which finally inhibited the growth of QBC939 cells.