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目的探讨转移抑制基因H1(NM23-H1)与多态性上皮黏蛋白1(MUC1)基因在甲状腺中的表达及临床意义。方法采用定量RT-PCR与图像扫描定量分析技术检测60例甲状腺癌中NM23-H1与MUC1基因的表达水平。结果 MUC1基因在甲状腺癌中表达率为75.0%(45/60);MUC1在无淋巴结转移组中的检出率为70.6%(24/34),在淋巴结转移组中的检出率为80.8%(21/26)(P>0.05)。NM23-H1基因在甲状腺癌中表达率为63.3%(38/60);NM23-H1在无淋巴结转移组中的检出率为73.5%(25/34),在淋巴结转移组中的检出率为50.0%(13/26)(P<0.01)。结论检测NM23-H1与MUC1基因的表达水平可能有助于判断甲状腺癌是否转移;NM23-H1低表达与MUC1高表达可能在甲状腺癌转移过程中具有内在联系。
Objective To investigate the expression of metastasis suppressor gene H1 (NM23-H1) and the polymorphism of epithelial mucin 1 (MUC1) gene in the thyroid and its clinical significance. Methods The expression of NM23-H1 and MUC1 in 60 thyroid carcinomas was detected by quantitative RT-PCR and image-scanning quantitative analysis. Results The positive rate of MUC1 in thyroid cancer was 75.0% (45/60). The positive rate of MUC1 in non-lymph node metastasis was 70.6% (24/34), and the positive rate of MUC1 in lymph node metastasis was 80.8% (21/26) (P> 0.05). The positive rate of NM23-H1 in thyroid cancer was 63.3% (38/60). The positive rate of NM23-H1 in non-lymph node metastasis was 73.5% (25/34), and the positive rate of NM23-H1 in lymph node metastasis Was 50.0% (13/26) (P <0.01). Conclusion Detecting the expression of NM23-H1 and MUC1 gene may be helpful in judging whether thyroid cancer metastasizes. The low expression of NM23-H1 and MUC1 may have an intrinsic relationship in the metastasis of thyroid cancer.