本文报道HPLC法测定生物体液中的头孢三嗪。提取方法分两步,首先在酸性条件下(PH=3)将药物提取至氯仿-正戊醇(3:1)混合液中,再在pH为7的环境中将药物从有机相提入水相。取10-50μl上清液进样。定量采用内标法(峰高比),内标物选用头孢唑肟。色谱柱为ODS—C18反相柱(250mmX4.6mm,5um),流动相为0.1M磷 This article reports the determination of ceftriaxone in biological fluids by HPLC. The extraction method consists of two steps: first, the drug is extracted into a chloroform-n-amyl alcohol (3: 1) mixture under acidic conditions (pH = 3); then, the drug is taken from the organic phase into the aqueous phase . Take 10-50μl of supernatant for injection. Quantitative internal standard method (peak height ratio), internal standard selection of ceftizoxime. Column ODS-C18 reverse phase column (250mmX4.6mm, 5um), the mobile phase 0.1M phosphorus