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本文采用微量细胞培养法观察细胞病变效应（Ｃｙｔｏｐａｔｈｉｃｅｆｅｃｔ，ＣＰＥ）测定经不同剂量空心莲子草作用后的病毒滴度，用逆转录－聚合物酶链反应（ＲＴ－ＰＣＲ）检测药物作用后细胞裂解液中的病毒核酸（ＣＶＢ３－ＲＮＡ），结果药物直接作用组和药物抗病毒吸附组均不能阻止病毒感染宿主细胞，药物抗病毒生物合成组则随培养液中药物浓度加大，细胞裂解液中病毒滴度下降，但ＣＶＢ３－ＲＮＡ却一直可检出。结论：①空心莲子草不能直接杀灭ＣＶＢ３；②空心莲子草不能阻止ＣＶＢ３吸附、穿入易感细胞；③空心莲子草可阻止ＣＶＢ３生物合成或／和以后环节发挥抗病毒作用
In this study, cytopathogenic effect (CPE) was used to measure the virus titer after different doses of Alternanthera philoxeroides, and cell lysate was detected by reverse transcription-polymerase chain reaction (RT-PCR). In the virus nucleic acid (CVB3-RNA), both the drug direct action group and the drug antiviral adsorption group could not prevent the virus from infecting the host cell, and the drug antiviral biosynthesis group increased the virus concentration in the cell lysate as the drug concentration in the culture medium increased. Titres decreased, but CVB3-RNA was consistently detectable. Conclusion: 1 Alternanthera philoxeroides can not directly kill CVB3; 2 Alternanthera philoxeroides can not prevent CVB3 from adsorbing and penetrating into susceptible cells; 3 Alternanthera philoxeroides can prevent CVB3 biosynthesis and/or play a role in antiviral action in later stages.