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[目的]探究 T USC3对肝癌细胞(SM MC‐7721细胞系)增殖、迁移和侵袭的影响.[方法]在SM MC‐7721细胞中转染T USC3过表达质粒,采用Real‐time PCR和Western blot技术检测 T USC3 mRNA和蛋白表达验证质粒转染效果. M T T检测转染后24 h 、48 h 、72 h和96 h时的细胞增殖情况,划痕实验检测细胞迁移,T ranswell小室检测细胞侵袭.[结果]本研究成功在SM MC‐7721细胞中过表达 T USC3 . M T T结果显示T USC3过表达转染组的细胞增殖能力显著低于空载体转染组( P ＜0 .01) .划痕实验结果显示T USC3过表达转染组的细胞迁移能力也显著下降( P ＜0 .05) .与划痕实验结果类似,T ranswell结果也表明T USC3过表达后,肝癌细胞侵袭能力下降( P ＜0 .01) .[结论]T USC3抑制人肝癌细胞SMMC‐7721细胞系的增殖、迁移和侵袭,可能在肝癌发展中发挥抑癌作用.“,”To investigate the effect of tumor suppressor candidate 3 (T USC3 ) on prolifera‐tion ,migration and invasion of hepatoma cells (SM MC‐7721 ).[M ethods]T USC3 overexpression vector was transfected into SM MC‐7721 cells ,and T USC3 mRNA and protein expression were detected by Real‐time PCR and Western blot to verify the transfection efficiency of plasmid .M T T was used to detect cell prolifera‐tion at 24 h ,48 h ,72 h and 96 h after transfection .T he cell migration ability was detected by wound healing and the invasion ability was detected by T ranswell chamber .[Results]T USC3 was overexpressed in SM MC‐7721 successfully .T he results of M T T showed that the cell proliferation in T USC 3‐overexpression group was significantly lower than that in empty vector group ( P ＜0 .01).T he results of the wound healing showed that the cell migration in the T USC3 overexpression transfection group was also significantly decreased ( P ＜0 .05).Similar to wound healing ,the results of T ranswell also showed that the invasive of hepatoma cells de‐creased after T USC3 overexpression ( P ＜0.01).[Conclusion] T USC3 could inhibit proliferation ,migration and invasion of hepatoma cells cells ,and may play a tumor suppressing role in the development of hepatic car‐cinoma .