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目的 检测涎腺多形性腺瘤中 p5 3基因突变。 方法 采用非同位素聚合酶链式反应———单股构型多态分析 (PCR -SSCP)技术对涎腺多形性腺瘤进行研究。结果 14例多形性腺瘤中有 11例出现PCR -SSCP异常电泳带 (阳性率 78.6 % )。突变部位分布在第 5～ 8外显子 ,其中第 5、6、7、8外显子的阳性例数分别为 2、6、7、3例。在 11例阳性肿瘤中有 2例肿瘤同时出现 3对外显子 (第 6、7、8外显子 )异常 ;3例肿瘤同时出现 2对外显子(第 6、7和 7、8外显子 )异常。结论 ①涎腺多形性腺瘤发生中 p5 3基因的第 5～ 8外显子均有突变 ,其中突变频率最高的是外显子 6和 7;②在阳性的肿瘤中约有半数出现多位点突变。
Objective To detect the mutation of p5 3 gene in salivary gland adenoma. Methods Polymorphic adenoma of salivary gland was studied by non-isotope polymerase chain reaction (PCR-SSCP) technique. Results In 14 cases of pleomorphic adenoma, 11 cases appeared PCR-SSCP abnormal electrophoresis band (positive rate 78.6%). The mutation sites were located in exon 5 to exon 5, of which the positive number of exon 5, 6, 7 and 8 were 2,6, 7 and 3, respectively. In 11 of the 11 positive tumors, 3 pairs of exons (6th, 7th, and 8th exons) were found simultaneously. In 3 cases, 2 pairs of exons (6th, 7th and 7th, 8th exons )abnormal. Conclusions ① There is a mutation in exon 5 ~ 8 of p5 3 gene in salivary gland adenoma, of which exons 6 and 7 are the most frequently mutated. ② About half of the positive tumors have multiple loci Point mutation.