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在室内测定菌株B215对香蕉枯萎病菌菌丝生长和孢子萌发都有良好抑制效果的基础上,优化拮抗菌株的最佳培养条件为:在NA培养基上,装液量为50mL/500mL,最适初始pH值为7.0～7.5,初始接菌量为1%,培养温度为28℃,以便更好地应用于田间试验。以此培养条件为依据,开展拮抗菌株的盆栽小苗实验,选用正交试验L8(27)设计,得出菌株B215对香蕉枯萎病菌的最佳防效组合为:在小苗定植前,用活菌培养液750mL/株浸根,可以降低小区香蕉枯萎病发病率。进一步试验验证结果显示:盆栽试验B215对Foc的防效为62.95%。
On the basis of good inhibition of mycelial growth and spore germination of Fusarium oxysporum f. Sp. In laboratory, the optimum culture conditions for optimizing the antagonistic strains were as follows: in NA medium, the liquid volume was 50mL / 500mL, the optimum The initial pH was 7.0-7.5, the initial amount of inoculation was 1% and the culture temperature was 28 ℃ for better application in field experiments. Based on this culture condition, pot experiment of antagonistic strains was carried out. The orthogonal experiment L8 (27) was designed and the best combination of strain B215 against Fusarium graminearum was obtained as follows: Liquid 750mL / strain soaking roots, can reduce the incidence of cell banana wilt disease. Further experimental verification results show that: pot experiment B215 Foc effect was 62.95%.