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目的探讨凋亡抑制基因Survivin在子宫内膜癌中的表达及其与P53,Ki-67基因表达的相关性。方法应用免疫组织化学链霉菌抗生物素蛋白-过氧化酶连接法(SP法)检测Survivin、P53、Ki-67基因在10例正常子宫内膜组织,及60例子宫内膜癌组织中的表达。结果Survivin基因在正常子宫内膜组织分泌期中见微弱表达,60例子宫内膜癌组织中,42例表达阳性,占70%。病理分级G1~G2级的子宫内膜癌的Sur-vivin基因表达阳性率为58.1%(18/31),G3级为82.75%(24/29),两者比较,差异有显著性(P<0.05)。临床分期Ⅰ期的子宫内膜癌的Survivin基因表达阳性率为3例中有1例,Ⅱ期者8例中有4例,Ⅲ期者为75.51%(37/49),3者比较,差异有显著性(P<0.05)。子宫肌层浸润深度<1/2的Survivin基因表达阳性率65.1%(28/43),>1/2的Survivin阳性率为76.5%(13/17),两者比较,差异有显著性(P<0.05)。子宫内膜癌中P53蛋白表达阳性、阴性中,Survivin基因表达阳性率分别为77.3%(34/44)、16例中有8例,两者比较,差异有显著性(P<0.05)。Ki-67蛋白表达阳性、阴性者中,Survivin基因表达阳性率分别为80.4%(37/46)、14例中有5例,两者比较,差异有显著性(P<0.005)。Survivin基因表达阳性率与子宫内膜癌组织中P53、Ki-67蛋白表达密切相关。结论Survivin基因可以作为评价子宫内膜癌的一项诊断指标,它的过度表达提示子宫内膜癌预后不良。Survivin基因过度表达抑制了细胞凋亡,促进细胞增殖,使Ki-67基因过度表达,而Ki-67又可作为子宫内膜癌5年生存率的一项评价指标。凋亡抑制基因Survivin的表达在子宫内膜癌中与P53、Ki-67蛋白的表达存在相关性。
Objective To investigate the expression of survivin gene in endometrial carcinoma and its relationship with the expression of P53 and Ki-67. Methods The expression of Survivin, P53 and Ki-67 in 10 cases of normal endometrium and 60 cases of endometrial carcinoma were detected by immunohistochemical streptavidin-peroxidase (SP method) . Results Survivin gene was weakly expressed in the secretory phase of normal endometrium. Of the 60 cases of endometrial carcinoma, 42 were positive, accounting for 70%. The positive rate of Sur-vivin gene expression was 58.1% (18/31) in G3 grade and 82.75% (24/29) in G3 grade in pathological grading G1-G2 grade, the difference was significant (P < 0.05). The positive rate of Survivin gene expression in stage Ⅰ endometrial cancer was 1 in 3 cases, 4 cases in stage Ⅱ and 75.51% (37/49) in stage Ⅲ, the difference was statistically significant There was significant (P <0.05). The positive rate of Survivin gene expression was 65.1% (28/43), and the positive rate of Survivin> 1/2 was 76.5% (13/17) in the depths of myometrial invasion less than 1/2. There was a significant difference between the two <0.05). The positive rates of Survivin gene expression in endometrial carcinoma were 77.3% (34/44) and 8 (16 cases), respectively. The difference was significant (P <0.05). The positive rate of Survivin gene expression was 80.4% (37/46) in the positive and negative cases of Ki-67, and 5 cases in 14 cases (P <0.005). The positive rate of Survivin gene expression is closely related to the expression of P53 and Ki-67 in endometrial carcinoma. Conclusion The Survivin gene can be used as a diagnostic indicator of endometrial cancer. The overexpression of Survivin gene may indicate the poor prognosis of endometrial carcinoma. Survivin gene overexpression inhibits apoptosis, promotes cell proliferation, and overexpression of Ki-67, which in turn can be used as an indicator of 5-year survival rate of endometrial cancer. The expression of Survivin, an apoptosis inhibitor, is correlated with the expression of P53 and Ki-67 in endometrial carcinoma.