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目的探讨支气管肺炎细菌感染的早期诊断方法。方法选取2010-09-01—2011-02-28长春市儿童医院住院的临床诊断支气管肺炎的患儿56例作为观察组,在16S rRNA基因保守区选出通用引物,对观察组患儿及对照组34例发热患儿的血液,采用聚合酶链反应(PCR)方法检测细菌16S rRNA基因。同时对两组患儿血液进行血培养、C反应蛋白(CRP)及白细胞(WBC)检测。结果用PCR方法扩增细菌,经电泳在相当于1300bp处均可见一DNA条带,而与病毒及人基因DNA无交叉反应。结论用PCR方法检测细菌16S rRNA基因具有较高的敏感性和特异性,优于血培养、WBC计数、CRP检测。对于支气管肺炎的患儿,16S rRNA基因检测能快速诊断是否为细菌感染。
Objective To investigate the early diagnosis of bronchial pneumonia bacterial infection. Methods selected from 2010-09-01-2011-02-28 Changchun Children’s Hospital inpatients with clinical diagnosis of bronchial pneumonia in 56 cases as observation group, 16S rRNA gene conservative region selected universal primers for observation group children and control Blood was collected from 34 children with fever and the bacterial 16S rRNA gene was detected by polymerase chain reaction (PCR). At the same time, blood culture, C-reactive protein (CRP) and white blood cell (WBC) were detected in two groups of children. Results The bacteria were amplified by PCR, and a DNA band was observed at the equivalent of 1300bp by electrophoresis, but no cross-reaction with virus and human DNA. Conclusion PCR detection of bacterial 16S rRNA gene has high sensitivity and specificity, better than blood culture, WBC count, CRP detection. For children with bronchopneumonia, 16S rRNA gene detection can quickly diagnose bacterial infections.