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目的研究骨巨细胞瘤(GCT)骨皮质破坏与肿瘤组织中P65、RANKL、OPG、MMP-9表达程度的关系,初步探讨MRI所示的侵袭性征象所代表的生物学行为。方法搜集2010年8月至2012年1月本院诊治的28例GCT患者,根据MRI表现有无骨皮质明显中断和软组织肿块形成分为2组,运用半定量逆转录聚合酶链式反应(RT-PCR)的方法检测各个肿瘤组织中P65、RANKL、OPG、MMP-9的mRNA的表达程度,分析2组间P65、RANKL、OPG、MMP-9的mRNA的表达程度有无差异以及各种蛋白的mRNA表达间有无相关性。所得的数据采用SPSS 13.0统计软件,采用秩和检验法和相关分析法,以P≤0.05为差异有统计学意义。结果有骨皮质明显中断和软组织肿块形成的GCT较无骨皮质明显中断和软组织肿块形成的GCT的P65、RANKL、MMP-9的表达量升高,OPG的表达无统计学差异。结论MRI表现有骨皮质中断征象的GCT,其骨质破坏蛋白表达增加,提示GCT中骨质破坏活动增加。
Objective To study the relationship between the destruction of cortical bone and the expression of P65, RANKL, OPG and MMP-9 in giant cell tumor of bone (GCT) and to explore the biological behavior represented by MRI. Methods Twenty-eight GCT patients diagnosed and treated in our hospital from August 2010 to January 2012 were divided into two groups according to the MRI findings of cortical bone disruption and soft tissue mass formation. Semi-quantitative reverse transcriptase-polymerase chain reaction The expression of P65, RANKL, OPG and MMP-9 mRNA in each tumor tissue was detected by RT-PCR and RT-PCR. The differences of mRNA expression of P65, RANKL, OPG and MMP- Whether there is a correlation between mRNA expression. The data obtained using SPSS 13.0 statistical software, using rank sum test and correlation analysis to P ≤ 0.05 for the difference was statistically significant. Results The expression of P65, RANKL and MMP-9 in GCT with obvious cortical bone cessation and soft tissue mass formation was significantly lower than that in non-cortical bone mass formation and soft tissue mass formation. The expression of OPG was not significantly different. Conclusions GCT with signs of cortical discomfort on MRI showed an increased expression of osteoclast proteins, suggesting an increase in bone destruction activity in GCT.