THE FUNCTIONAL ROLE OF 23 kD AND 24 kD POLYPEPTIDES OF THYLAKOID IN Mg~(2+)-INDUCED CHANGE OF EXCITA

来源 :Science in China,Ser.B | 被引量 : 0次 | 上传用户:hawk1918
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In contrast to the chloroplasts from higher plants, Mg~(2+)-induced PS--Ⅱ fluorescence inten-sity increase does not relate to Mg~(2+)-induced surface charge density decrease of thylakoidin the chloroplasts from Codium fragile. Tbe extraction of the green alga chloroplasts withCa~(2+) to remove the 30--31kD polypeptide (Q_B protein) on the thylakoid surface does notaffect the above Mg~(2+)-induced phenomena. If the Ca~(2+)-treated chloroplasts are further di-gested by trypsin to remove the 23kD and 24kD polypeptides on the membrane surface,the Mg~(2+)-induced fluorescence effect will completely disappear whereas the property ofMg~(2+)-induced surface charge density changes remains unchanged. These results not onlyshow that the 23kDa and 24kDa polypeptides on the thylakoid surface are the specific act-ing sites of the cation that induce Chla fluorescence change, but also demonstrate that thecation-induced change of excitation energy distribution between two photosystems is not con-trolled by the electros In contrast to the chloroplasts from higher plants, Mg ~ (2 +) - induced PS - Ⅱ fluorescence inten sity increase does not relate to Mg ~ (2 +) - induced surface charge density decrease of thylakoidin the chloroplasts from Codium fragile. Tbe extraction of the green alga chloroplasts with Ca ~ (2+) to remove the 30 - 31 kD polypeptide (Q_B protein) on the thylakoid surface does not affect the above Mg ~ (2 +) - induced phenomena. ) -treated chloroplasts are further di-gested by trypsin to remove the 23 kD and 24 kD polypeptides on the membrane surface, the Mg ~ (2 +) - induced fluorescence effect will completely disappear while the property of Mg ~ (2 +) - induced surface charge density changes remains unchanged. These results not onlyshow that the 23kDa and 24kDa polypeptides on the thylakoid surface are the specific act-ing sites of the cation that induce Chla fluorescence change, but also demonstrate that thecation-induced change of excitation energy distribution between two photosystems is not con-trol led by the electros
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