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原肌球蛋白Tropomyosin是虾蟹类的主要致敏物质,除此以外在其他方面功能的研究非常少。本研究克隆了克氏原螯虾Tropomyosin基因,进行了同源序列比对,并分析了Tropomyosin基因在克氏原螯虾卵巢发育过程中的转录水平表达模式。研究结果显示,Tropomyosin蛋白序列高度保守,在虾蟹类甲壳动物中,8个抗原决定簇序列几乎完全一致。本研究克隆的基因存在3个核苷酸位置(c61—t,g118—a,c359—t)的变化,但是均不处于抗原决定簇内,免疫原性不受影响。Tropomyosin基因mR NA在克氏原螯虾卵巢组织中表达量很低,但是随着卵巢的发育存在表达差异,即呈现一个先提高再降低的峰型特征。本研究暗示Tropomyosin除过敏原特性外,可能也受性腺发育的部分调控影响。
Tropomyosin, the main sensitizing substance of shrimp and crab, has very few studies on other aspects of its function. In this study, we cloned the Tropomyosin gene from Procambarus clarkii and compared it with the homologous sequence, and analyzed the transcriptional level of Tropomyosin during ovary development of Procambarus clarkii. The results show that Tropomyosin protein sequence is highly conserved, in the shrimp and crab crustaceans, the eight antigenic determinant sequence is almost exactly the same. The cloned gene in this study has three nucleotide positions (c61-t, g118-a, c359-t) changes, but are not within the epitope, the immunogenicity is not affected. The mR NA of Tropomyosin gene is very low in ovarian tissue of Procambarus clarkii, but with the difference in the expression of ovary, there is a peak-type characteristic of increasing and then decreasing. This study suggests that Tropomyosin may be partially regulated by gonadal development in addition to allergen properties.