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从来源于普通小麦品种济南 1 77(Triticumaestivumcv .Jinan 1 77)悬浮细胞系的原生质体与来源于簇毛麦 (Haynaldiavillosa)胚性愈伤组织的原生质体融合获得体细胞杂种。供体簇毛麦原生质体在融合之前用紫外线照射 3 0s或 1min ,紫外线剂量为 3 6 0 μW /cm2 。仅由紫外线照射 3 0s的组合获得再生愈伤组织克隆。细胞学、生物化学及PCR分析结果证实了再生克隆的杂种性质。用线粒体基因特异的探针进行的RFLP分析的结果表明 ,杂种中含有融合双亲的线粒体并且发生了重组。由杂种愈伤组织再生得到白化苗。讨论了紫外线对融合产物的影响。
Somatic hybrids were obtained by fusion of protoplasts derived from the suspension cell line of common wheat variety Triticum aestivum cv. Jinan 1 77 with protoplasts derived from embryogenic callus of Haynaldia villosa. The protoplasts of donor H. rhamnosus were irradiated with UV light for 30 s or 1 min before the fusion and the UV dose was 3600 μW / cm 2. Regenerated callus clones were obtained only by a combination of 30 s of UV irradiation. Cytology, biochemistry and PCR analysis confirmed the hybrid nature of regenerated clones. The results of RFLP analysis using mitochondrial gene-specific probes showed that the hybrids contained the mitochondria of the confluent parents and recombination occurred. Albino seedlings are regenerated from hybrid callus. The effect of UV on the fusion product was discussed.