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目的探讨多种mi RNA(mi R-29a、mi R-99a、mi R-127、mi R-199a)在高危人乳头瘤病毒(HPV)感染的宫颈组织中的表达情况,探讨其在宫颈癌发病过程中的作用。方法采用Real-time PCR检测高危HPV感染的宫颈组织中的四种mi RNA的表达及其与高危HPV感染的宫颈组织各级临床病理改变之间的关系。结果与正常组相比mi R-29a在宫颈上皮内瘤变(CIN)Ⅰ组、CINⅡ组和宫颈癌组中表达下调,差异有统计学意义(P<0.05)。mi R-99a、mi R-199a各级别宫颈病变中均表达下调,差异有统计学意义(P<0.05)。mi R-127在CINⅠ组和CINⅡ组中表达差异无统计学意义,而CINⅢ组和宫颈癌组中表达下调,差异有统计学意义(P<0.05)。结论四种mi RNA在高危HPV感染所致的各级CIN及宫颈癌病变中表达下降,mi RNA可能与高危HPV所致的CIN向宫颈癌的衍变中发挥作用。
Objective To investigate the expression of multiple miRNAs (mi R-29a, mi R-99a, mi R-127, mi R-199a) in cervical cancer tissues infected with high-risk human papillomavirus (HPV) The role of the disease process. Methods Real-time PCR was used to detect the expression of four miRNAs in high-risk HPV-infected cervical tissues and their relationship with the clinicopathological changes of cervical tissues at high-risk HPV infection. Results Compared with normal group, mi R-29a expression was down-regulated in CIN group Ⅰ, CINⅡ group and cervical cancer group (P <0.05). Mi R-99a, mi R-199a all levels of cervical lesions were down-regulated, the difference was statistically significant (P <0.05). The expression of mi R-127 in CINⅠgroup and CINⅡgroup was not statistically significant, while in CINⅢgroup and cervical cancer group, the difference was statistically significant (P <0.05). Conclusions The expression of four miRNAs in all stages of CIN and cervical cancer caused by high-risk HPV infection is decreased. MiRNA may play a role in the evolution of CIN caused by high-risk HPV to cervical cancer.