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目的：观察血性脑脊液对内皮细胞分泌内皮素（Ｅｎｄｏｔｈｅｌｉｎ，ＥＴ）和一氧化氮（Ｎｉｔｒｉｃ Ｏｘｙｄｅ，ＮＯ）的影响。方法：建立脐静脉内皮细胞体外培养的方法，用放射免疫方法测定ＥＴ含量，用硝酸还原酶法测定ＮＯ的代谢产物ＮＯ_２～－／ＮＯ＿３～－（ＮＯ＿ｘ～－）含量，并观察血性脑脊液的作用。结果：不同时间血性脑脊液均使内皮细胞ＥＴ分泌增加，ＮＯ分泌减少。结论：血性脑脊液可使体外培养的内皮细胞ＥＴ分泌增加，ＮＯ分泌减少。
Objective: To observe the effect of bloody cerebrospinal fluid (CSF) on the secretion of endothelin (ET) and nitric oxide (NO) in endothelial cells. Methods: The culture method of umbilical vein endothelial cells was established in vitro. The contents of ET were measured by radioimmunoassay. The content of NO 2 - / NO 3 - - (NO x -) was determined by nitrate reductase method and the effect of bloody cerebrospinal fluid . Results: Cerebrospinal fluid (CSF) at different times increased ET secretion and decreased NO secretion. Conclusion: Cerebrospinal fluid can increase the secretion of endotheliocyte ET in vitro and reduce the secretion of NO.