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采用饲毒烟蚜(MyzuspersicaeSulzer)和汁液摩擦传毒的方法,对22种烟蚜田间常见的中间寄主进行接种,接种后7d,用无毒烟蚜接种中间寄主饲养2d后回接到健康烟株。观察烟株是否发病。经多次重复试验,最终确定小酸浆(PhysalisangulataL)和龙葵(DaturastramoniumL)为烟草丛顶病的中间寄主。采用汁液传毒中间寄主并用蚜虫回接烟苗未出现发病症状。
The common intermediate hosts of 22 kinds of aphids were inoculated by the method of rubbing and poisoning by the Myzus persicae sulzer and the juice. After 7 days of inoculation, the intermediate host was inoculated with a non-toxic aphid and reared in the healthy host . Observe the incidence of tobacco plants. After many repeated experiments, Physalisangulata L and Daturastramonium L were finally identified as intermediate hosts of tobacco bushy disease. Infected by the intermediate host juice and aphids back to tobacco seedlings did not appear symptoms.