采用计算机图像定量测量方法,对高压氧中毒(HBOI)后小鼠软脑膜微血管和大脑皮质毛细血管内皮细胞碱性磷酸酶(ALP)活性进行了观察和定量测量.结果表明,HBOI后小鼠软脑膜毛细血管开放数目减少,毛细血管密度降低.毛细血管内皮细胞ALP活性增强,微血管呈扩张状态,管周有渗出,血流速度变慢,有红细胞聚集现象.这些变化可能是活性氧对血管内皮细胞的毒性作用所致.作者认为,脑的微循环障碍和毛细血管内皮细胞ALP活性增强可能是氧中毒发病的重要机理之一. The alkaline phosphatase (ALP) activity of capillary endothelial cells in the pia mater and cortex of mice after hyperbaric oxygen poisoning (HBOI) was observed and quantified by computer image quantitative measurement method.The results showed that the mouse soft The number of capillaries of the meninges was decreased and the density of capillaries was decreased.The ALP activity of capillary endothelial cells was enhanced, the state of microvessels was dilated, the perirenal exudate, the blood flow slowed down and erythrocytes aggregated.These changes may be the result of reactive oxygen species Endothelial cells caused by toxic effects.The authors believe that the brain microcirculation and capillary endothelial cell ALP activity may be one of the important pathogenesis of oxygen poisoning.